Molecular Techniques and Methods

Transformation of Plant Protoplasts

Copy Right © 2001/ Institute of Molecular Development LLC

INTRODUCTION

Methods of DNA delivery to protoplasts include;
(1) infection of protoplast-derived cells with intact Agrobacteria,
(2) chemically stimulated uptake of isolated DNA into protoplasts,
(3) fusion of bacterial spheroplasts with protoplasts, and
(4) fusion and uptake of liposomes carrying DNA into protoplasts.

Transformants can be selected by the tumorous character of growth substance independence, or the antibiotic resistance conferred by foreign genes (e.g., kanamycin resistance).




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