Molecular Techniques and Methods

1 M Tris-HCl Buffer (pH 7.0-8.8)

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Trizma base
121.1 g
Distilled H2O
800 ml
conc. HCl
40-70 ml

  • Adjust the pH to 7.0-8.8 with conc. HCl.
  • Add distilled H2O to make a final volume of 1 liter.
  • Dispense into aliquots and sterilize by autoclaving.

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