Molecular Techniques and Methods
1 M Tris-HCl Buffer (pH 7.0-8.8)
Copy Right © 2001/ Institute of Molecular Development LLC
| Trizma base |
121.1 g
|
| Distilled H2O |
800 ml
|
| conc. HCl |
40-70 ml
|
Adjust the pH to 7.0-8.8 with conc. HCl.
Add distilled H2O to make a final volume of 1 liter.
Dispense into aliquots and sterilize by autoclaving.