Molecular Techniques and Methods

20 X SSPE (1 liter)

Copy Right © 2001/ Institute of Molecular Development LLC

3 M NaCl
175.3 g
0.2 M NaH2PO4.H2O
27.6 g
20 mM Na-EDTA.2H2O
7.5 g
Add distilled H2O to make a final volume of
1 liter

  • Adjust the pH to 7.4 with 12-14 ml, 5N NaOH solution.
  • Dispense into aliquots.
  • Sterilize by autoclaving.


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