Molecular Techniques and Methods
20 X SSPE (1 liter)
Copy Right © 2001/ Institute of Molecular Development LLC
3 M NaCl
175.3 g
0.2 M NaH
2
PO
4
.H
2
O
27.6 g
20 mM Na-EDTA.2H
2
O
7.5 g
Add distilled H
2
O to make a
final volume
of
1 liter
Adjust the pH to 7.4 with 12-14 ml,
5N NaOH solution
.
Dispense into aliquots.
Sterilize by autoclaving.
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