Molecular Techniques and Methods

Phenol: Chloroform: IAA (25: 24: 1)

Copy Right © 2001/ Institute of Molecular Development LLC

Phenol
25 ml
Chloroform
24 ml
IAA (Isoamylalcohol)
1 ml

  • Equilibrate the mixture by extracting 3-5 times with 0.1 M Tris-HCl (pH 7.6) or TE (pH 7.6).
  • Store the equilibrated mixture under an equal volume of 0.01 M Tris-HCl (pH 7.6) at 4oC in dark glass bottles.



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