In this procedure, guanidine thiocyanate is used to lyse cells
and to denature proteins including ribonucleases. Most polysaccharides
that remain in the aqueous phase are selectively bound by cellulose.
RNA is further purified from residual contaminants by lithium
In this procedure, the yield of total RNA is 2 ug/mg of cell suspension culture.
MATERIALS AND SOLUTIONS
Decontaminates all materials and solutions from RNase contamination accoring to the Protocol.