M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase)
is an RNA-dependent DNA polymerase requiring a DNA primer and
a RNA template to synthesize a complementary DNA strand. The enzyme
is a product of the pol gene of M-MuLV and consists of a single
subunit with a molecular weight of 7l kDa. M-MuLV RT has a weaker
intrinsic RNase H activity than Avian Myeloblastosis Virus (AMV)
reverse transcriptase which is important for achieving long full-length
complementary DNA (>7kb).
5 X Reaction Buffer
250 mM Tris-HCl (pH 8.3)
375 mM KCl
15 mM MgCl
2
50 mM DTT
REFERENCES
Tanes, N, Goff, SP (1998) PNAS 85: 1977.
Roth, M. et. al. (1985) J. Biol. Chem. 260: 9326.