Exonuclease-free Klenow Fragment is a genetically engineered Klenow
DNA polymerase from which the 3' to 5' exonuclease activity has
been removed, leaving only the polymerase activity. The lack of
exonuclease activity makes Exo
- Klenow Fragment the enzyme of choice for isotopic or biotin labeling
of DNA probes by the random-primed method, and for DNA sequencing
by the Sanger dideoxy method.
10 X Reaction Buffer
500 mM Tris-HCl (pH 7.2)
100 mM MgSO
4 1 mM DTT