Molecular Techniques and Methods

DNA Polymerase I Large Fragment
(Klenow Fragment)

Copy Right © 2001/ Institute of Molecular Development LLC

The DNA Polymerase I Large (Kienow) Fragment lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I but retains the 5' to 3' polymerase, the 3' to 5' exonuclease and strand displacement activities.




10 X Reaction Buffer
500 mM Tris-HCl (pH 7.2)
100 mM MgSO4
1mM DTT




REFERENCES
  • Joyce, C.M. and Grindley, N.D.F. (1983) PNAS 80: 1830.



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