Molecular Techniques and Methods

T4 Polynucleotide Kinase

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T4 Polynucleotide Kinass catalyzes the transfer of the -phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 3'-phosphate group.




10 X Reaction Buffer
700 mM Tris-HCl (pH 7.6)
100 mM MgCl2
50 mM DTT




REFERENCES
  • Richardson, C.C. (1965) PNAS 54: 158-163.



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