Molecular Techniques and Methods

T4 Polynucleotide Kinase

Copy Right © 2001/ Institute of Molecular Development LLC

T4 Polynucleotide Kinass catalyzes the transfer of the -phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 3'-phosphate group.

10 X Reaction Buffer
700 mM Tris-HCl (pH 7.6)
100 mM MgCl2
50 mM DTT

  • Richardson, C.C. (1965) PNAS 54: 158-163.

  • Please send your comment on this protocol to "".

  • "Return to Molecular Techniques and Methods"

    "Return to Homepage"