RNasins Ribonuclease Inhibitor has broad spectrum RNase inhibitory
properties including the inhibition of eukaryotic RNases of the
neutral type. The 50 kDa protein exerts its inhibitory effect
by binding noncovalently to RNases in a 1:1 ratio with an association
constant greater than 1014. Inhibits common eukaryotic RNases including RNase A, RNase B,
RNase C and human placental RNase. Does not inhibit RNase H, S1
Nuclease, SP6, T7 or T3 RNA Polymerase, AMV or M-MuLV Reverse
Transcriptase, Taq DNA Polymerase, RNase T1. Active over a broad
Useful in any applications where eukaryotic RNase contamination
is a potential problem. Protection of mRNA in cDNA synthesis reactions.
In vitro transcription/translation systems.
Increased yields and activity of polysomes.
Improvement of in vitro virus replication.
Improvement of RNA translation in homologous systems.
Preparation of RNase-free antibody.
Blackburn, P, Moore, S (1982) In: "The Enzymes", Vol. XV, Part
Blackburn, P, Wilson, S, Moore, S (1977) J. Biol. Chem.