Molecular Info®

Molecular Info® Copy Right © 2001 Institute of Molecular Development LLC

Cloning of a Gene Cloning by mRNA Differential Display Cloning by 5'-RACE Cloning by 3'-RACE Cloning by Genome Walking cDNA Library Construction Screening Lamda Phage cDNA Library Screening Lamda Phage cDNA Expression Library PCR Screening of Lamda Phage cDNA Library DNA Excision from Lamda ZAP Vectors Genomic Library Construction Lamda Phage Vector Preparation for Genomic Library Construction Screening Genomic DNA Library Construction of Combinatorial Peptide Library Construction of Phage Display PeptideLibrary Screening of a Combinatorial Peptide Library Screening of a Phage Display Peptide Library YAC Library Construction Screening of Yeast Two-Hybrid Library Yac Library Screening Electrophoresis Agarose Gel Electrophoresis of DNA DNA Fingerprinting by RFLP and RAPD Genomic DNA Fingerprinting by Pulsed Field Gel Electrophoresis Polyacrylamide Gel Electrophoresis of DNA Preparation of Sequencing Gels Silver staining of DNA Formaldehyde Gel Electrophoresis of RNA Deionization of Formaldehyde, Formamide, and Glyoxal by Mixed Bed Resin Deionization of Formaldehyde, Formamide, and Glyoxal by AG 501-X8 Mixed Bed Resin Native Gel Electrophoresis of Protein SDS-PAGE of Protein Acetic acid-Urea PAGE of Protein 2-D Gel Electrophoresis of Protein Starch Gel Electrophoresis of Protein Coomassie Blue Staining of Protein Silver Staining of Protein Isolation and Manipulation of Nucleic Acids Modification Enzymes Restriction Enzymes DNA Purification from Agarose Gels Removal of RNA from DNA Samples Isolation of Chromosomal DNA from Animal Cells Isolation of Mitochondrial DNA from Animal Cells Isolation of Nuclear DNA from Animal Cells Isolation of Chromosomal DNA from E. coli Isolation of Plasmid DNA Preparation of Phage Lamda DNA Isolation of Yeast DNA Isolation of Fungal DNA Genomic DNA Isolation from Plant Tissues Isolation of Chloroplast and Chloroplast DNA Isolation of Mitochondrial DNA from Cell Suspension Cultures Isolation of Mitochondrial DNA from Plant Tissues Isolation of Mitochondrial DNA from Green Fruits Isolation of Mitochondria for in Organello Protein Synthesis Isolation of Nuclear DNA from Plant Tissues Small Scale Preparation of Genomic DNA from Plant Tissues for PCR Isolation of Poly A+-RNA Isolation of total RNA from paraffin-embedded tissues Removal of DNA from RNA sample Removal of RNase contamination and DEPC-treatment Analysis of RNA quality Isolation of Mitochondrial RNA from Animal Cells Isolation of Total RNA from Animal Cells Isolation of Total RNA from Blood Isolation of Total RNA from Paraffin-Embedded Tissues Isolation of RNA for mRNA Differential Display Isolation of RNA using RNAzol Isolation of Total RNA from Bacterial Cells Isolation of Total RNA from Plant Cells Isolation of Total RNA from Plant Cell Suspension Isolation of Polysomal RNA from Plant Tissues Isolation of Mitochondrial RNA from Plant Cells Isolation of Total RNA from Paraffin-embedded Tissues Protein Expression, Purification, and Identification Native Gel Electrophoresis of Protein SDS-PAGE of Protein Acetic acid-Urea PAGE of Protein 2-D Gel Electrophoresis of Protein Starch Gel Electrophoresis of Protein Coomassie Blue Staining of Protein Silver Staining of Protein In vitro Translation Using Wheat Germ Isolation of Mitochondria for in Organello Protein Synthesis Processing of Fusion Proteins Purification of Recombinant Proteins from Inclusion Bodies Purification of Recombinant Proteins Tagged with Affinity Handles Purification of Soluble Recombinant Proteins Purification of Surfactant Protein B Fusion Protein Preparation from Lamda gt11 Phages Protein Purification by Gel Filtration Chromatography Protein Purification by Immunoaffinity Chromatography Radiolabeled Protein Purification by Immunoprecipitation with Antibody-Sepharose Unlabeled Protein Purification by Immunoprecipitation with Antibody-Sepharose Protein Purification by Ion-Exchange Chromatography Protein Purification by Ion-Exchange HPLC Protein Purification by RP-HPLC Protein Purification by Size-Exclusion HPLC Desalting and Concentration of Dilute Proteins Degassing Water, Buffer and Solvent UV Cutoffs of Solvents Physical Properties of Solvents Purification of DNA-Binding Proteins Using Biotin-Streptavidin Affinity Systems Purification of DNA-Binding Proteins Using Biotin-Cellulose Microcolumn Ammonium Sulfate Saturation Table Protein Quantitation by Bradford Method The Lowry Method for Protein Quantitation Protein Quantitation by BCA Assay Identification of Unknown Protein by Mass Spectrometer Analysis Polymerase Chain Reaction PCR Long Distance PCR PCR Primers UV Irradiation for De-Contamination RT-PCR Quantitative RT-PCR Semi-Quantitative RT-PCR: Competitive RT-PCR Semi-Quantitative RT-PCR: Noncompetitive RT-PCR In situ PCR In situ RT-PCR PCR in situ Hybridization Probe Generation and Labeling Hybridoma Production for Monoclonal Antibody Production Immuno Screening of Hybridomas Purification of Monoclonal Antibodies Production of Polyclonal Antibodies Nonradioactive Labeling of 3'-end Oligonucleotides by Terminal Transferase Nonradioactive Labeling of DNA by Nick Translation Nonradioactive Labeling of DNA by PCR Nonradioactive Labeling of DNA by random priming Nonradioactive Tailling of 3'-end Oligonucleotides by Terminal Transferase Estimating the Yields of Dig-labeled Nucleic Acids RNA Labeling by In vitro transcription 3'-end Labeling of DNA with Klenow Fragment 3'-end Labeling of Oligonucleotides with Terminal Transferase 5'-end Labeling of DNA with T4 Polynucleotide Kinase DNA Labeling by Nick Translation DNA Labeling by PCR DNA Labeling by Random Priming Labeled Single stranded cDNA Synthesis from mRNA Removal of Unincorporated dNTPs RNA Labeling by In vitro Transcription Promoter and Genome Analysis DNaseI Finger Printing Gel Mobility Shift Assay for DNA Binding Proteins Using Low-Ionic-Strength PAGE Gel Mobility Shift Assay for DNA Binding Proteins Using High-Ionic-Strength PAGE Purification of DNA Binding Proteins Using Biotin-Streptavidin Affinity Systems Purification of DNA-Binding Proteins Using Biotin-Cellulose Microcolumn Genome Walking by Inverse Polymerase Chain Reaction AFLP (Amplified Fragment Length Polymorphisms) RAPD (Random Amplified Polymorphic DNA) Genomic Fingerprinting by Pulsed Field Gel Electrophoresis In vitroMutagenesis Site-directed In vitro Mutagenesis with Oligonucleotides Site-specific In vitro Mutagenesis by Chemical Modifications Gene Targeting by Homologous Recombination Deletion Mutagenesis by Overlap Extension and Splicing PCR Short Sequence-Insertion Mutagenesis by PCR Long Sequence-Insertion Mutagenesis using PCR-Generated Sticky-Feet Random Mutagenesis by PCR Sequencing Cycle Sequencing of Purified Plasmid DNA Cycle Sequencing of Bacterial Colonies Containing High-Copy-Number Plasmids Cycle Sequencing of Bacterial Colonies Containing Low-Copy-Number Plasmids Cycle Sequencing of M13, Cosmids, and Lamda DNA Cycle Sequencing of Bacterial Genomic DNA Cycle Sequencing of PCR Products Cycle Sequencing of Genomic DNA Cycle Sequencing by Highly Degenerate Primers Preparation of Sequencing Gels Chemical Sequencing of DNA Dideoxy Chain-Termination Sequencing of plasmid DNA Dideoxy Chain-Termination Sequencing of PCR products Signal Transduction In vivo Protein Phosphorylation in Mammalian Cells In vitro Protein Phosphorylation in Mammalian Cell Extracts In vivo Protein Phosphorylation in Plants In vitro Protein Phosphorylation in Plant Cell Extracts Signal Detection DNA-DNA Hybridization Alkali Blotting of DNA for Hybridization Tissue Fixation FAA Fixation of Plant Tissue Glutaraldehyde-Osmium Fixation of Plant Tissue Glutaraldehyde-Paraformaldehyde Fixation of Plant Tissue Paraffin Infiltration and Sectioning of Plant Tissue Staining Paraffin Sections Staining Paraffin Sections with Safranin O Staining Paraffin Sections with Safranin-Fast Green Staining Paraffin Sections with Toluidine Blue In situ Hybridization to Metaphase Chromosomes Detection of DNA in Paraffin Embedded Tissue Sections Identification of Bacterial Cells by In situ Hybridization PCR In situ Hybridization In situ PCR In situ Hybridization on Paraffin Embedded Tissues WISH (Whole mount In Situ Hybridization) In situ RT-PCR Western Transfer Blotting Coomassie Blue Staining of Protein Silver Staining of Protein Northern Blot Analysis Ribonuclease Protection PCR-RPA RT-PCR In situ RT-PCR Nuclear Run-On Analysis Chemiluminescent Detection of Digoxigenin-Labeled Probe Transformation Calcium Phosphate-Mediated Transformation High Efficiency Calcium Phosphate-Mediated Transformation Gene Transfer by electroporation Gene Transfer by lipofection Gene Transfer by SV40 vectors Transformation by protoplast fusion Gus Reporter Gene Expression in animal tissue One Step Transformation of E. coli Transformation of E. coli Preparation of Competent E.coli cells Transformation of Frozen competent cells Triparental conjugation Insert Screening Cloned in E. coli by PCR Arabidopsis Transformation by Vacuum Infiltration Leaf Disc Transformation Alfalfa Leaf Disc Transformation Tobacco Leaf Disc Transformation Protoplast Preparation Preparation and Culture of Protoplasts from a Sterile Shoot Culture, and Regeneration of Plants Preparation and Culture of Leaf Mesophyll Protoplasts from Greenhouse Grown Plants Preparation and Culture of Protoplasts from Roots of Phaseolus aureus (Mung Bean) Isolation and Culture of Protoplasts from a Cell Suspension Culture of Daucus carota (Carrot) Preparation and Culture of Protoplasts from a Nonmorphogenic Suspension Culture of a Graminaceous Species Lolium multiflorum Protoplast Transformation Transformation of Protoplast-Derived Cells with Agrobacterium Transformation of Protoplast by Chemically Stimulated Uptake of Isolated DNA Transformation of Protoplasts by Fusion of Bacterial Spheroplasts Transformation of Protoplasts by Liposome-Encapsulated Delivery of DNA Gene Targeting in Plants Gus Reporter Gene expression in plants Localization of GUS Reporter Gene Expression in Plants Protein Targeting Yeast Spheroplast Transformation Yeast Transformation by Lithium Chloride Biochemical Methods Enzyme assay of UDP-glucuronosyltransferase Preparation of Glutamate from Cell Culture Preparation of Lactate from Cell Culture Preparation of Fatty Acids from Cell Culture Cell Biology Isolation of Nuclei from Mammalian Cells Isolation of Microsomes from Mammalian Cells Isolation of Mitochondria from Mammalian Cells Isolation of Nuclei from Plant Tissue Isolation of Microsomes from Plant Tissue Isolation of Microsomes from Fungi Isolation of Chloroplasts Isolation of Mitochondria from Plant Tissue Isolation of Cell Walls from Plant Tissue Flow Cytometric Analysis of Isolated Nuclei from Plant Tissue Miscellaneous Information/ Buffers/ Culture Media Biotech Company Addresses Environmentally Friendly Cocktails Radioactive Waste Control Disposal of Ethidium Bromide TE Buffer STE Buffer STET Buffer TNT Buffer Electrophoresis Buffers Enzyme Buffers Gel Loading Buffers LB Medium NZCYM Medium NZYM Medium NZM Medium Terrific Broth SOB Medium SOC Medium 2 X YT Medium M9 Minimal Medium NZY Medium 20% Maltose and 1 M Maltose SM TM Lamda Diluent Physical Properties of Solvents UV Cutoffs of Solvents Stock Solutions 30% Acrylamide:Bis Solution 40% Acrylamide:Bis Solution 10 M Ammonium Acetate 10% Ammonium Persulfate Antibiotic Solutions 0.1 M ATP BCIP 2 X BES-Buffered Saline 1 M CaCl2 2.5 M CaCl2 100 mM dNTPs 1 M Dithiothreitol (DTT) 0.5 M EDTA (pH 8.0) Ethidium Bromide (10 mg/ml) 2 X HEPES-Buffered Saline IPTG 1 M Magnesium Acetate 1 M MgCl2 2-Mereaptoethanol (BME) NBT Phenol Preparation Phenol: Chloroform 10 mM Phenylmethylsulfonyl Fluoride (PMSF) Phosphate-Buffered Saline (PBS) 1 M Potassium Acetate (pH 7.5) Potassium Acetate (for Alkaline Lysis) 3 M Sodium Acetate (pH 5.2) 3 M Sodium Acetate (pH 7.0) 5 M NaCl 10% Sodium Dodecyl Sulfate (SDS) 20 X SSC 20 X SSPE Trichloroacetic Acid (TCA)-100% Solution 1 M Tris-HCl Tris-Buffered Saline (TBS) (25 mM Tris) X-Gal Chloroform: IAA 50% Triton X-100 1 M MgSO4 10 ug/ul Glycogen 1 M ZnCl2 1 M Spermidine 0.1 M HEPES/ 1 M HEPES 1 M KCl 5 M NaOH 5% Xylene Cyanol 5% BromoPhenol Blue 10 M LiCl 100 mM NTPs 1 M Sodium Citrate 10% Sarkosyl 50% Nonidet P40 0.5 M EGTA 1 M NaH2PO4 50% Glycerol 100% Denhardt's Solution Salmon Sperm DNA (10 mg/ml) 1 M PIPES (pH 6.8) Proteinase K (20 mg/ml) 1 M Sodium-Phosphate Buffer Pronase (20 mg/ml) RNase A (10 mg/ml) DNase (10 mg/ml) 1 M Tricine-NaOH (pH 8.0-7.0) 1 M Phosphoric Acid 1 M Tris-Acetate (pH 8.0-7.0) 1 M MnCl2 0.5 M Tris-Phosphate (pH 6-8) 1 M Glycine-NaOH Buffer (pH 10) Concentrations of Common Commercial Acids and Bases 1 M (NH4)2SO4 100 mM rATP 1% Gelatin 10% Tween-20 1% Acetylated BSA (10 mg/ml) 1% Toluidine Blue 1% Pepsin 10% Blocking Reagent 3% NiCl2 Torula Yeast RNA (10 mg/ml) 1% Coomassie Brilliant Blue R