Molecular Techniques and Methods

Plasmid Transformation into E.coli Competent Cells


Copy Right © 2001/ Institute of Molecular Development LLC



INTRODUCTION




MATERIALS AND SOLUTIONS

LB containg 10 mM MgCl2




PROCEDURES


1. Add 10-100 ng plasmid DNA to 50 - 100 ul E.coli competent cells.

2. Incubate in ice for 20-40 min.

3. Treat cells at 37-42oC for 40 sec - 1 min.

4. Immediately, put tube in ice for 1 min.

5. Add 500 ul LB containing 10 mM MgCl2.

6. Incubate 1 hour in 37oC incubator, 250 rpm.

7. Plate on LB+antibiotics plates.

8. Colony forms in overnight incubation.



NOTES



KIT INFORMATION



REFERENCES


Please send your comment on this protocol to "editor@MolecularInfo.com".

Home
MT&M
Online Journal
Hot Articles
Order Products
Classified