Molecular Techniques and Methods

Plasmid Transformation into E.coli Competent Cells Prepared by RbCl Method


Copy Right © 2001/ Institute of Molecular Development LLC



INTRODUCTION




MATERIALS AND SOLUTIONS

Psi Broth (100 ml)
LB Medium
4 mM MgSO4
10 mM KCl

  • Autoclave.




    PROCEDURES

    1. Add 2 ul of ligation mix to 100 ul E.coli competent cells.

    2. Keep on ice for 20 min.

    3. Transfer the tube to a 42oC water bath for 90 sec.

    4. Add 500 ul Psi-broth.

    5. Incubate for 90 min in 37oC incubator, 250 rpm.

    6. Plate cells on LB+antibiotics plates.




    NOTES




    KIT INFORMATION


    REFERENCES


    Please send your comment on this protocol to "editor@MolecularInfo.com".

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