Molecular Techniques and Methods

Staining Paraffin Sections with Safranin O

Copy Right © 2001/ Institute of Molecular Development LLC

INTRODUCTION




MATERIALS AND SOLUTIONS

Safranin Staining Solution (10 ml)
2% Safranin O ---------------------------- 0.2 g
95% Ethyl alcohol ------------------------- 10 ml




PROCEDURES

1. Dry paraffin embeded sections at 60oC overnight.

2. Deparaffinize slides in xylene.

3. Cover the sections with several drops of Safranin Staining Solution.

4. Rinse with distilled H2O and air-dry.

5. Mount with a suitable medium.




NOTES




KIT INFORMATION




REFERENCES

  • Berlyn GP, Miksche JP (1976) Botanical Microtechnique and Cytochemistry, Ames, Iowa, Iowa State University Press.

  • Please send your comment on this protocol to "editor@MolecularInfo.com".

  • Home
    MT&M
    Online Journal
    Hot Articles
    Order Products
    Classified