Molecular Techniques and Methods

UV Cutoffs for Solvent,
Wavelength Cutoffs for Common Mobile Phases

Copy Right © 2001/ Institute of Molecular Development LLC

Solvent UV Cutoffs

Solvent
UV Cutoffs
Acetonitrile
190 nm
Amyl alcohol
210 nm
Acetone
330 nm
Amyl chloride
225 nm
Benzene
280 nm
Cyclohexane
200 nm
Cyclopentane
200 nm
Carbon disulfide
380 nm
Carbon tetracholoride
265 nm
Chloroform
245 nm
Diethyl amine
275 nm
Ethanol
210 nm
Ethylene glycol
210 nm
2-Butoxyethanol
220 nm
p-Dioxane
215 nm
Ethyl acetate
256 nm
Ethylene dichloride
230 nm
Ethyl ether
220 nm
Ethyl sulfide
290 nm
Isopropyl ether
220 nm
Isopropyl chloride
225 nm
Isopropanol
205 nm
Methanol
205 nm
Methylene chloride
233 nm
Methyl-isobutyl ketone
334 nm
Methyl ethyl ketone
330 nm
1-Nitropropane
380 nm
Nitromethane
380 nm
Octane
215 nm
n-Pentane
190 nm
Petroleum ehter
210 nm
n-Propanol
210 nm
n-Propyl chloride
225 nm
Pyridine
330 nm
Toluene
285 nm
Tetrahydrofuran
230 nm
Xylene
290 nm




Wavelength Cutoffs for Common Mobile Phases

Mobile Phase
UV Cutoff
1% Acetic acid
230 nm
10 mM Ammonium bicarbonate
190 nm
0.1% BRIJ 35
190 nm
0.1% CHAPS
215 nm
50 mM Diammonium phosphate
230 nm
10 mM Ammonium acetate
205 nm
1 mM Na2-EDTA
190 nm
0.1% HCl
190 nm
10 mM HEPES (pH 7.6)
225 nm
10 mM MES (pH 6.0)
225 nm
10 mM Potassium phosphate-monobasic
190 nm
10 mM Potassium phosphate-dibasic
230 nm
PIC Reagent B-6 (1 vial/liter)
225 nm
PIC Reagent D-4 (1 vial/liter)
190 nm
PIC Reagent A (1 vial/liter)
< 200 nm
1 M NaCl
208 nm
10 mM Sodium citrate
225 nm
10 mM Sodium acetate
205 nm
0.1% SDS
190 nm
10 mM Sodium formate
200 nm
0.1% Trifluoroacetic acid (TFA)
205 nm
0.1% Triethylamine
235 nm
0.15 Tween 20
190 nm
0.1% Triton-X 100
240 nm
20 mM Tris-HCl (pH 7.0)
204 nm
20 mM Tris-HCl (pH 8.0)
212 nm

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