Molecular Techniques and Methods
2 x BES-Buffered Saline (100 ml)
Copy Right © 2001/ Institute of Molecular Development LLC
BES (N,N-bis[2-hydroxy-ethyl]-2-aminoethanesulfonic acid)
0.5 g
NaCl
1.6 g
Na
2
HPO
4
0.027 g
Add distilled H
2
O to make a
final volume
of
100 ml
Adjust the pH to 7 with 1 M HCl.
Sterilize the solution by passage through a 0.22 um filter.
Store in aliquots at -20
o
C.
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