Molecular Techniques and Methods
10% Blocking Reagent
Copy Right © 2001/ Institute of Molecular Development LLC
0.1 M Maleic acid
5 ml of 1 M Maleic acid
0.15 M NaCl
1.5 ml of 5 M NaCl
0.175 M NaOH
1.75 ml of 5 M NaOH
Add distilled H
2
O to make a
final volume
of
40 ml
Adjust the pH to 7.5 with conc. NaOH.
Blocking Reagent
5 g
Add distilled H
2
O to make a
final volume
of
50 ml
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