Molecular Techniques and Methods

10% Blocking Reagent

Copy Right © 2001/ Institute of Molecular Development LLC

0.1 M Maleic acid
5 ml of 1 M Maleic acid
0.15 M NaCl
1.5 ml of 5 M NaCl
0.175 M NaOH
1.75 ml of 5 M NaOH
Add distilled H2O to make a final volume of
40 ml
  • Adjust the pH to 7.5 with conc. NaOH.
  • Blocking Reagent
    5 g
    Add distilled H2O to make a final volume of
    50 ml




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