Molecular Techniques and Methods

RNase A (10 mg/ml)

Copy Right © 2001/ Institute of Molecular Development LLC

RNase A
100 mg
50 mM Tris-HCl (pH 7.5)
500 ul of 1 M Tris-HCl
15 mM NaCl
30 ul of 5 M NaCl
100% Glycerol
5 ml
Distilled H2O
4.5 ml

  • Heat to 100oC for 15 minutes.
  • Cool slowly to room temperature
  • Dispense into aliquotes and store at -20oC.



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