Molecular Techniques and Methods

1 M Sodium Phosphate Buffer (pH 5.8-8.0)

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pH
1 M Na2HPO4
1 M NaH2PO4
8.0
93.2 ml
6.8 ml
7.8
89.6 ml
10.4 ml
7.6
84.5 ml
15.5 ml
7.4
77.4 ml
22.6 ml
7.2
68.4 ml
31.6 ml
7.0
57.7 ml
42.3 ml
6.8
46.3 ml
53.7 ml
6.6
35.2 ml
64.8 ml
6.4
25.5 ml
74.5 ml
6.2
17.8 ml
82.2 ml
6.0
12.0 ml
88.0 ml
5.8
7.9nml
92.1 ml




1 M Na2HPO4 (100 ml)
Na2HPO4
14.2 g
Add distilled H2O to make a final volume of
100 ml



1 M NaH2PO4 (100 ml)
NaH2PO4
12 g
Add distilled H2O to make a final volume of 100 ml




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