Molecular Techniques and Methods

Phosphate-Buffered Saline
(PBS)

Copy Right © 2001/ Institute of Molecular Development LLC

10x PBS

1.4 M NaCl
80 g
27 mM KCl
2 g
100 mM Na2HPO4
14.4 g
18 mM KH2PO4
2.4 g
Add distilled H2O to make a final volume of
1 liter




1x PBS

140 mM NaCl
8 g
2.7 mM KCl
0.2 g
10 mM Na2HPO4
1.44 g
1.8 mM KH2PO4
0.24 g
Add distilled H2O to make a final volume of
1 liter

  • Adjust the pH to 7.4 with HCl.
  • Autoclave solution.
  • Store at room temperature.



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