Molecular Techniques and Methods

40% Acrylamide:Bis Solution
(19:1) (29:1) (37.5:1)

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40% Acrylamide:Bis Solution (19:1)
Acrylamide
38 g
N,N'- methylenebisacrylamide
2 g
Add distilled H2O to make a final volume of
100 ml



40% Acrylamide:Bis Solution (29:1)
Acrylamide
29 g
N,N'- methylenebisacrylamide
1 g
Add distilled H2O to make a final volume of
75 ml



40% Acrylamide:Bis Solution (37.5:1)
Acrylamide
37.5 g
N,N'- methylenebisacrylamide
1 g
Add distilled H2O to make a final volume of
96.25 ml

  • Heat the solution to 37oC to dissolve the chemicals.
  • Filter the solution through a 0.45 um-membrane filter.
  • Adjust the pH to less than 7.0.
  • Store the solution in dark bottles at room temperature for less than 3 months.



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