Molecular Techniques and Methods
2 x HEPES-Buffered Saline (100 ml)
Copy Right © 2001/ Institute of Molecular Development LLC
NaCl
1.6 g
KCl
0.074 g
Na
2
HPO
4
.2H
2
O
0.027 g
Dextrose
0.2 g
HEPES
1 g
Add distilled H
2
O to make a
final volume
of
100 ml
Adjust the pH to 7.0 with 0.5 N NaOH.
Sterilize the solution by passage through a 0.22 um filter.
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