Molecular Techniques and Methods

2 x HEPES-Buffered Saline (100 ml)

Copy Right © 2001/ Institute of Molecular Development LLC

NaCl
1.6 g
KCl
0.074 g
Na2HPO4.2H2O
0.027 g
Dextrose
0.2 g
HEPES
1 g
Add distilled H2O to make a final volume of
100 ml

  • Adjust the pH to 7.0 with 0.5 N NaOH.
  • Sterilize the solution by passage through a 0.22 um filter.



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