Molecular Techniques and Methods

0.5 M EDTA (pH 8.0) (1 liter)

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EDTA.2H2O-Na2 (Ethylenediaminetetraacetate.2H2O-disodium salt)
186.1 g
Add distilled H2O to make a final volume of
1 liter

  • Adjust the pH to 8.0 with NaOH (i.e., 20 g of NaOH pellets).
  • Dispense into aliquots and sterilize by autoclaving.

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