Molecular Techniques and Methods
0.5 M EDTA (pH 8.0) (1 liter)
Copy Right © 2001/ Institute of Molecular Development LLC
EDTA.2H
2
O-Na
2
(Ethylenediaminetetraacetate.2H
2
O-disodium salt)
186.1 g
Add distilled H
2
O to make a
final volume
of
1 liter
Adjust the pH to 8.0 with NaOH (i.e., 20 g of NaOH pellets).
Dispense into aliquots and sterilize by autoclaving.
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