Molecular Techniques and Methods

100 mM Deoxyribonucleoside Triphosphates (dNTPs)

Copy Right © 2001/ Institute of Molecular Development LLC

1. Dissolve each dNTP in 10 mM Tris-HCl (pH7.0) at a concentration of 200 mM.

2. Dilute an aliquot of the dNTP appropriately, and read the optical density at the wavelengths given in the table below.

3. Calculate the actual concentration of each dNTP.
(For a cuvette with a path length of 1 cm,
Absorbance = e/M.)

4. Dilute the solutions with H2O to a final concentration of 100 mM dNTP.

5. Store each separately at -70oC in small aliquots.


Base Wavelength (nm) Extinction Coeffecients [e(M-1 cm-1)]
A 259 1.54 X 104
G 253 1.37 X 104
C 271 9.10 X 103
T 260 7.40 X 103
U 262 1.00 X 104



Home
MT&M
Online Journal
Hot Articles
Order Products
Classified