Molecular Techniques and Methods

SM Buffer

Copy Right © 2001/ Institute of Molecular Development LLC

This buffer is used, for storage and dilution of bacteriophage A stocks.


5 M NaCl
20 ml
1 M MgSO4
8.5 ml
1 M Tris-HCl (pH 7.5)
50 ml
1% Gelatin Solution
10 ml
Add distilled H2O to make a final volume of
1 L

  • Dispense 50 ml aliquots and sterilize the buffer by autoclaving for 20 minutes at 15 lb/ in2.
  • Store at room temperature.


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