Molecular Techniques and Methods
SM Buffer
Copy Right © 2001/ Institute of Molecular Development LLC
This buffer is used, for storage and dilution of bacteriophage A stocks.
5 M NaCl
20 ml
1 M MgSO
4
8.5 ml
1 M Tris-HCl
(pH 7.5)
50 ml
1% Gelatin Solution
10 ml
Add distilled H
2
O to make a final volume of
1 L
Dispense 50 ml aliquots and sterilize the buffer by autoclaving for 20 minutes at 15 lb/ in
2
.
Store at room temperature.
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