Molecular Techniques and Methods

2 x YT Medium
(2 x TY Medium)

Copy Right © 2001/ Institute of Molecular Development LLC

Bacto-tryptone
16 g
Bacto-yeast extract
10 g
NaCl
5 g
Add distilled H2O to make a final volume of
1 L
  • Adjust the pH to 7.0 with 5 N NaOH.

  • Sterilize the medium by autoclaving for 20 minutes at 15 lb/ in2.





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