Molecular Techniques and Methods
2 x YT Medium
(2 x TY Medium)
Copy Right © 2001/ Institute of Molecular Development LLC
Bacto-tryptone
16 g
Bacto-yeast extract
10 g
NaCl
5 g
Add distilled H
2
O to make a
final volume
of
1 L
Adjust the pH to
7.0
with 5 N NaOH.
Sterilize the medium by autoclaving for 20 minutes at 15 lb/ in
2
.
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