Molecular Techniques and Methods
SOB Medium
Copy Right © 2001/ Institute of Molecular Development LLC
Bacto-Tryptone
20 g
Bacto-Yeast Extract
5 g
NaCl
0.5 g
1 M KCl
2.5 ml
Add distilled H
2
O to make a
final volume
of
1 L
Adjust the pH to 7.0 with 5 N NaOH (0.2 ml).
Sterilize by autoclaving for 20 minutes at 15 lb/ in
2
.
Just before use,
add 10 ml of a sterile solution of 1 M MgCl
2
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