Molecular Techniques and Methods

SOB Medium

Copy Right © 2001/ Institute of Molecular Development LLC

Bacto-Tryptone
20 g
Bacto-Yeast Extract
5 g
NaCl
0.5 g
1 M KCl 2.5 ml
Add distilled H2O to make a final volume of
1 L

  • Adjust the pH to 7.0 with 5 N NaOH (0.2 ml).
  • Sterilize by autoclaving for 20 minutes at 15 lb/ in2.

  • Just before use, add 10 ml of a sterile solution of 1 M MgCl2



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