Molecular Techniques and Methods

Terrific Broth
(Tartof and Hobbs, 1987)

Copy Right © 2001/ Institute of Molecular Development LLC

KH2PO4-K2HPO4 Solution
KH2PO4
2.31 g
K2HPO4
12.54 g
Add distilled H2O to make a final volume of
100 ml
  • Sterilize by autoclaving for 20 minutes at 15 lb/ in2.



    Terrific Broth
    Bacto-Tryptone
    12 g
    Bacto-Yeast Extract
    24 g
    Glycerol
    4 ml
    Add distilled H2O to make a final volume of
    900 ml

  • Mix well.
  • Sterilize by autoclaving for 20 minutes at 15 lb/ in2.
  • Allow the solution to cool to 60oC or less.
  • Add 100 ml of sterile KH2PO4-K2HPO4 solution.



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