Molecular Techniques and Methods
Terrific Broth
(Tartof and Hobbs, 1987)
Copy Right © 2001/ Institute of Molecular Development LLC
KH
2
PO
4
-K
2
HPO
4
Solution
KH
2
PO
4
2.31 g
K
2
HPO
4
12.54 g
Add distilled H
2
O to make a
final volume
of
100 ml
Sterilize by autoclaving for 20 minutes at 15 lb/ in
2
.
Terrific Broth
Bacto-Tryptone
12 g
Bacto-Yeast Extract
24 g
Glycerol
4 ml
Add distilled H
2
O to make a
final volume
of
900 ml
Mix well.
Sterilize by autoclaving for 20 minutes at 15 lb/ in
2
.
Allow the solution to cool to 60
o
C or less.
Add 100 ml of sterile KH
2
PO
4
-K
2
HPO
4
solution.
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