Molecular Techniques and Methods
LB Medium
(Luria-Bertani Medium)
Copy Right © 2001/ Institute of Molecular Development LLC
Bacto-Tryptone
10 g
Bacto-Yeast extract
5 g
NaCl
10 g
Add distilled H
2
O to make a
final volume
of
1 L
Add 15 g bactoagar for LB plate.
Adjust the pH to 7.0 with 5 N NaOH (0.2 ml).
Sterilize by autoclaving for 20 minutes at 15 lb/ in
2
.
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