Molecular Techniques and Methods

LB Medium
(Luria-Bertani Medium)

Copy Right © 2001/ Institute of Molecular Development LLC

Bacto-Tryptone
10 g
Bacto-Yeast extract
5 g
NaCl
10 g
Add distilled H2O to make a final volume of
1 L

  • Add 15 g bactoagar for LB plate.
  • Adjust the pH to 7.0 with 5 N NaOH (0.2 ml).
  • Sterilize by autoclaving for 20 minutes at 15 lb/ in2.


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