Molecular Techniques and Methods
5 x Tris-Glycine-SDS Electrophoresis Buffer
Copy Right © 2001/ Institute of Molecular Development LLC
Tris Base
15.1 g
Glycine (pH 8.3)
94 g
10% SDS
50 ml
Add distilled H
2
O to make a
final volume
of
1 L
Autoclave and store at room temperature.
For SDS-polyacrylamide gels electrophoresis - use 1 x Tris-Glycine Buffer.
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