Molecular Techniques and Methods

5 x TBE Electrophoresis Buffer

Copy Right © 2001/ Institute of Molecular Development LLC

Tris Base
54 g
Boric acid
27.5 g
0.5 M EDTA (pH 8.0)
20 ml
Add distilled H2O to make a final volume of
1 L

  • Autoclave and store at room temperature.
  • Discard any batches that develop a precipitate.

  • For Agarose gel electrophoresis - use 0.5 x TBE Buffer
  • For Polyacrylamide gel electrophoresis - use 1 x TBE Buffer


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