Molecular Techniques and Methods

Restriction Enzymes

Copy Right © 2001/ Institute of Molecular Development LLC


(N = A, C, G, or T)

Enzyme
Restriction Sequence
Enzyme
Restriction Sequence
Aat I AGGCCT Aat II GACGTC
Acc I GT(A/C)(G/T)AC Acc II CGCG
Acc III TCCGGA Afl III AC(A/G)(C/T)GT
Age I ACCGGT Aha II G(A/G)CG(C/T)C
Alu I AGCT Alw I GGATC
Aos II G(A/G)CG(C/T)C Apa I GGGCCC
ApaL I GTGCAC Apy I CC(A/T)GG
Ase I ATTAAT Asp700 I GAANNNNTTC
Asp7l8 I GGTACC Asu II TTCGAA
Ava I C(C/T)CG(A/G)G Ava II GG(A/T)CC
Avi II AGCGCT Bal I TGGCCA
BamH I GGATCC Ban I GG(C/T)(A/G)CC
Ban II G(A/G)GC(C/T)C Ban III ATCGAT
Bbi II G(A/G)CG(C/T)C BbrP I CACGTG
Bbs I GAAGAC Bbu I GCATGC
Bbv I GC(A/T)GC Bcl I TGATCA
Bcn I CC(G/C)GG Bfr I CTTAAG
Bgl II AGATCT Bna I GGATCC
Bsa I GGTCTC BsaA I (C/T)ACGT(A/G)
BsaB I GATNNNNATC Bsm I GAATGC
BsmA I GTCTC Bsp106 I ATCGAT
Bgl I GCCNNNNNGGC Bspl286 I G(A/G/T)GCHC
BspH I TCATGA BspM I ACCTGC
BspM II TCCGGA BssH II GCGCGC
Bst I GGATCC BstB I TTCGAA
BstE II GGTNACC BstN I CC(A/T)GG
BstU I CGCG BstX I CCANNNNNNTGG
BstY I (A/G)GATC(C/T) BsuE I CGCG
BsuF I CCGG BsuM I CTCGAG
BsuQ I CCGG BsuR I GGCC
Ccr I CTCGAG Cto I GCGC
Cfr I (C/T)GGCC(A/G) Cfr6 I CAGCTG
Cfr9 I CCCGGG Cfr10 I (A/G)CCGG(C/T)
Cfrl3 I GGNCC Cla I ATCGAT
Cpe I TGATCA Csp I CGG(A/T)CCG
Csp45 I TTCGAA CviJ I (A/G)GC(C/T)
CviP I CC Dde I CTNAG
Dpn I GmATC Dpn II GATC
Dra I TTTAAA Dra II (A/G)GGNCC(C/T)
Eae I (C/T)GGCC(A/G) Eag I CGGCCG
Ear I GAAGAG EclX I CGGCCG
Eco47 I GG(A/T)CC Eco47 III AGCGCT
EcoA GAGNNNNNNNGTCA EcoB TGANNNNNNNNTGCT
EcoD XXI TCANNNNNNNAATC EcoE GAGNNNNNNNATGC
EcoK AACNNNNNNGTGC EcoO109 I (A/G)GGNCC(C/T)
EcoR I GAATTC EcoR II CC(A/T)GG
EcoR V GATATC EcoR 124 GAANNNNNN(A/G)TCG
EcoR 124/3 GAANNNNNNN(A/G)TCG Ehe I GGCGCC
Esp I GCTNAGC Fnu4H I GCNGC
FnuD II CGCG Fok I CATCC
Fse I GGCCGGCC Fsp I TGCGCA
Hae II (A/G)GCGCY Hae III GGCC
Hap II CCGG Hga I GACGC
HgiA I G(A/G)GC(C/T)C HgiC I GG(C/T)(A/G)CC
HgiC II GG(A/T)CC HgiE I GG(A/T)CC
HgiJ II GG(C/T)(A/G)CC Hha I GCGC
Hha II GANTC Hinc II GT(C/T)(A/G)AC
Hind II GT(C/T)(A/G)AC Hind III AAGCTT
Hinf I GANTC HinP I GCGC
Hpa I GTTAAC Hpa II CCGG
Hph I TCACC Kpn I GGTACC
Kpn2 I TCCGGA Ksp I CCGCGG
Mae II ACGT Mam I GATNNNNATC
Mbo I GATC Mbo II GAAGA
Mfl I (A/G)GATC(C/T) Mlu I ACGCGT
Mme II GATC Mnl I CCTC
Mro I TCCGGA Mse I TTAA
Msp I CCGG Mst II CCTNAGG
Mun I CAATTG Mva I CC(A/T)GG
Mvn I CGCG Nae I GCCGGC
Nar I GGCGCC Nci I CC(G/C)GG
Nco I CCATGG Nde I CATATG
Nde II GATC Nhe I GCTAGC
Nla III CATG Not I GCGGCCGC
Nru I TCGCGA Nsi I ATGCAT
Nsp I (A/G)CATG(C/T) NspB II C(A/C)GC(G/T)G
PflM I CCANNNNNTGG Pfu I CGTACG
PaeR7 I CTCGAG Pml I CACGTG
PpuAI CGTACG PspA I CCCGGG
Pst I CTGCAG Pvu I CGATCG
Pvu II CAGCTG Rsa I GTAC
Rsr I GAATTC Rsr II CGG(A/T)CCG
Sac I GAGCTC Sac II CCGCGG
Sal I GTCGAC Sau3A I GATC
Sau96 I GGNCC Sca I AGTACT
ScrF I CCNGG SfaN I GATGC
Sfi I GGCCNNNNNGGCC Sfl I CTGCAG
SgrA I CRCCGG(C/T)G Sin I GGWCC
Sma I CCCGGG SnaB I TACGTA
Sno I GTGCAC Spe I ACTAGT
Sph I GCATGC Spl I CGTACG
Spo I TCGCGA Srf I GCCC/GGGC
Ssp I AATATT Sst I GAGCTC
Stu I AGGCCT StySP I AACNNNNNNGT(A/G)C
Taq I TCGA Taq II GACCGA or, CACCCA
Tfi I GA(A/T)TC Tha I CGCG
Xba I TCTAGA Xho I CTCGAG
Xho II (A/G)GATCPy Xma I CCCGGG
Xma III CGGCCG Xmn I GAANNNNTTC
Xor II CGATCG

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