Molecular Techniques and Methods

DNA Polymerase I

Copy Right © 2001/ Institute of Molecular Development LLC

DNA Polymerase I catalyzes the polymerization of nucleotides into duplex DNA in a 5' to 3' direction. Like many other DNA polymerases, the enzyme possesses a 3' to 5' exonuclease activity or "proofreading" function. DNA Polymerase I also contains a 5' to 3' exonuclease activity, enabling the enzyme to replace nucleotides in the growing strand of DNA.




10 X Reaction Buffer
500 mM Tris-HCl (pH 7.2)
100 m MgSO4
l mM DTT




REFERENCES
  • Protocols and Applications Guide, Third Edition (1996) Promega Corporation.



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