Molecular Techniques and Methods
Taq DNA Polymerase
Copy Right © 2001/ Institute of Molecular Development LLC
Taq DNA Polymerase is a thermostable enzyme that replicates DNA at 74
C and remains functional even after incubation at 95
C. This unmodified enzyme has an apparent molecular weight of 94,000 daltons.
10 X Reaction Buffer
500 mM KCl
100 mM Tris-HCl(pH 9.0)
1.0 % Tritons X-100
15 mM MgCl
Buffer is optimized for use with 0.2 mM for each of the dNTPs.
Chien, A et aL (1976) J. Bacteriol. 127: 1550.
Kaledin, AS et aL (1980) Biokhimiia 45: 644.
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