Molecular Techniques and Methods
DNase I (RNase Free)
Copy Right © 2001/ Institute of Molecular Development LLC
Catalyzes the degradation of double-stranded DNA into oligonucleotides and mononucleotides DNase I was isolated as a mixture of four isoenzymes that cut preferentially next to pyrimidine nucleotides
10 X Reaction Buffer
400 mM Tris-HCl (pH 7.5)
60 mM MgCl
20 mM CaCl
Laskowsld, M., Sr. (1971) In The Enzymes (Boyer, P.D., ed) Vol. 4, p.289. Academic Press, New York.
Moore, S. (1981) In The Enzymes (Boyer, P.D., ed) Vol. 14, p.281. Academic Press, New York
Please send your comment on this protocol to "