Molecular Techniques and Methods

DNase I (RNase Free)

Copy Right © 2001/ Institute of Molecular Development LLC


Catalyzes the degradation of double-stranded DNA into oligonucleotides and mononucleotides DNase I was isolated as a mixture of four isoenzymes that cut preferentially next to pyrimidine nucleotides




10 X Reaction Buffer
400 mM Tris-HCl (pH 7.5)
60 mM MgCl2
20 mM CaCl2




REFERENCES

  • Laskowsld, M., Sr. (1971) In The Enzymes (Boyer, P.D., ed) Vol. 4, p.289. Academic Press, New York.

  • Moore, S. (1981) In The Enzymes (Boyer, P.D., ed) Vol. 14, p.281. Academic Press, New York


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