Molecular Techniques and Methods

T3 RNA Polymerase

Copy Right © 2001/ Institute of Molecular Development LLC

T3 RNA Polymerase is a DNA-dependent RNA polymerase which exhibits extremely high specificity for T3 promoter sequences. The enzyme will incorporate 32P, 35S and 3H-labeled nucleotide triphosphates.




5 X Buffer
200 mM Tris-HCl (pH 7.9)
30 mM MgCl2
10 mM Spermidine
50 mM NaCl



  • Please send your comment on this protocol to "editor@MolecularInfo.com".

  • "Return to Molecular Techniques and Methods"

    "Return to Homepage"