Molecular Techniques and Methods

T4 DNA Ligase

Copy Right © 2001/ Institute of Molecular Development LLC

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and 3?-hydroxyl groups of adjacent nucleotides in either a blunt-end or cohesive-end configuration. The enzyme has been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule, but will not join single-stranded nucleic acids.

10 X Reaction Buffer
300 mM Tris-HCl (pH 7.8)
100 mM MgCl2
100 mM DTT
10 mM ATP.
  • This buffer should be stored frozen at -20oC in small aliquots to preserve the ATP.

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