Pfu DNA polymerase, derived from the hyperthermophilic archae
Pyrococcusfuriosus, has been shown to exhibit superior thermostability and proofreading
properties compared to other thermostable polymerases. Unlike
Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses
3' to 5' exonuclease proofreading activity that enables the polymerase
to correct nucleotide-misincorporation errors. This means that
Pfu DNA polymerase-generated PCR fragments will have fewer errors
than Taq-generated PCR inserts. Using Pfu DNA polymerase in PCR
reactions results in blunt-ended PCR products, which are ideal
for cloning into blunt-ended vectors, such as the PCR-Script-vectors.
Pfu DNA polymerase is an ideal choice for techniques that require
high-fidelity DNA synthesis.
10 x Reaction Buffer
100 mM KCl 100 mM (NH4)2SO4
200 mM Tris-HCl (pH8.7) 20 mM MgSO4 1% Triton X-100 1 mg/ ml BSA