AMV Reverse Transcriptase

AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is a DNA polymerase that uses DNA, RNA or RNA: DNA hybrids as a template. It requires a primer and Mg²⁺ or Mn²⁺ for activity. The avian enzyme possesses an intrinsic ribonuclease H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.




5 X REACTION BUFFER
250 mM Tris-HCl (pH 8.3)
250 mM KCl
50 mM MgCl₂
2.5 mM Spermidine
50 mM DTT.




REFERENCES

Pfeffer, D, Mierendorf, R. (1987) Meth. Enzymol. 152: 563.

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