Molecular Techniques and Methods

Deionization of Formamide, Formaldehyde, and Glyoxal
by AG 501-X8 Mixed Bed Resin

Copy Right © 2001/ Institute of Molecular Development LLC

INTRODUCTION

The rapid oxidation of formamide, formaldehyde, and glyoxal requires deionization prior to use for optimal efficacy in hybridization and nucleic acid denaturation applications. AG 501-X8 resin is a 1:1 equivalent mixture of AG 1-X8 resin (OH-) and AG 50W-X8 resin (H+).




MATERIALS AND SOLUTIONS

AG 501-X8 mixed bed resin (Bio-Rad).




PROCEDURES

1. Add 1 g of AG 501 -X8 resin to each gram of material to be deionized.

2. Mix the solution by magnetic stirrer for 1 hour.

3. Filter twice through Whatman No. 1 filter paper.

4. Dispense into 1 ml aliquotes and store at -20oC.

  • Once opened, the unused portion of a previously deionized aliquot should be discarded.





    • NOTES




    KIT INFORMATION




    REFERENCES


  • Please send your comment on this protocol to "editor@MolecularInfo.com".
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