Molecular Techniques and Methods

Deionization of Formamide, Formaldehyde, and Glyoxal
by Mixed Bed Resin

Copy Right © 2001/ Institute of Molecular Development LLC

INTRODUCTION

The rapid oxidation of formamide, formaldehyde, and glyoxal requires deionization prior to use for optimal efficacy in hybridization and nucleic acid denaturation applications. Mixed bed resin is strongly acidic and strongly basic, facilitating rapid deionization.




MATERIALS AND SOLUTIONS

Mixed bed resin (Sigma; Catalog No. M-8032)




PROCEDURES

1. Mix 50 ml of the reagent to be deionized with 5 g of mixed bed resin.

2. Mix the solution by magnetic stirrer for 30 min.

3. As the capacity of the resin is reached, its color will change from blue to amber.

4. Filter (or centrifuge) to remove the resin.

5. Store deionized glyoxal in tightly sealed, small aliquots at -20oC.
  • Deionized formamide and formaldehyde should be used shortly after deionization.
  • Once opened, the unused portion of a previously deionized aliquot should be discarded.






    • NOTES




    KIT INFORMATION




    REFERENCES


  • Please send your comment on this protocol to "editor@MolecularInfo.com".
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